B7-H4 Recombinant Protein
B7-H4 Recombinant Protein
Product Specifications
Product Name Alternative
V-set Domain-containing T Cell Activation Inhibitor 1, VTCN1, B7h.5, Immune Costimulatory Protein B7-H4, T Cell Costimulatory Molecule B7x, Protein B7S1
UniProt
P16410
Tag
Fc Tag
Source
CHO cells
Field of Research
Immunology & Inflammation
Purity
>98% (SDS-PAGE) . Endotoxin level is less than 0.06EU/ μg protein (LAL test; Lonza) .
Bioactivity
Measured by the ablility to inhibit anti-CD3-induced proliferation of stimulated human T cells. Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well place coated with 500 ng/ml anti-CD3 and 10ug/ml human B7H4/Fc. The presence of human B7H4/Fc at 10ug/ml inhibited anti CD3 response by 30-50%. Optimal dilutions should be determined by each laboratory for each application.
Form
Lyophilized
Storage Conditions
Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Notes
For research use only.
Applications Notes
This recombinant proteins is for research use only.
NCBI Accession Number
NP_078902
Preservative
Lyophilized from 0.2um-filtered solution in PBS. Reconstitute with 100 μl sterile water. Add 1X PBS to the desired protein concentration.
AA Sequence
The extracellular domain of human B7-H4 (aa 29-258) is fused to the N-terminus of the Fc region of human IgG1.
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