CHRNA4 (aa29-43) Antibody
Goat polyclonal antibody to CHRNA4
Product Specifications
Product Name Alternative
Anti CHRNA4 antibody, anti cholinergic receptor, nicotinic, alpha 4 antibody, anti BFNC antibody, anti EBN antibody, anti EBN1 antibody, anti NACRA4 antibody, anti cholinergic receptor, nicotinic, alpha 4 subunit antibody, anti cholinergic receptor, nicotinic, alpha polypeptide 4 antibody, anti neuronal acetylcholine receptor alpha 4 subunit antibody, anti neuronal nicotinic acetylcholine receptor alpha-4 subunit antibody
Reactivity
Human, Rat
Target
CHRNA4 (aa29-43)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
HVETRAHAEERLLKK
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:64000, WB: 1-3 μg/ml
Molecular Weight
70
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 70kDa band observed in Rat Brain lysates (calculated MW of 70.0kDa according to Human NP_000735.1and of 70.2kDa according to Rat NP_077330.1) . Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Bovine, Canine, Mouse
Tested Applications
ELISA, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
1137
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