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CHRNA4 (aa29-43) Antibody

Goat polyclonal antibody to CHRNA4

Product Specifications

Product Name Alternative

Anti CHRNA4 antibody, anti cholinergic receptor, nicotinic, alpha 4 antibody, anti BFNC antibody, anti EBN antibody, anti EBN1 antibody, anti NACRA4 antibody, anti cholinergic receptor, nicotinic, alpha 4 subunit antibody, anti cholinergic receptor, nicotinic, alpha polypeptide 4 antibody, anti neuronal acetylcholine receptor alpha 4 subunit antibody, anti neuronal nicotinic acetylcholine receptor alpha-4 subunit antibody

Reactivity

Human, Rat

Target

CHRNA4 (aa29-43)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

HVETRAHAEERLLKK

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:64000, WB: 1-3 μg/ml

Molecular Weight

70

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 70kDa band observed in Rat Brain lysates (calculated MW of 70.0kDa according to Human NP_000735.1and of 70.2kDa according to Rat NP_077330.1) . Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Bovine, Canine, Mouse

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

1137

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