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Cathepsin B Recombinant Protein

Cathepsin B Recombinant Protein

Product Specifications

Product Name Alternative

CTSB, CPSB, APPS

UniProt

P07858

Tag

His Tag

Source

HEK293 cells

Field of Research

Cancer Biology, Neuroscience, Signal Transduction

Purity

>95% as determined by SDS-PAGE.

Bioactivity

Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC. Measured in 100μl reaction mixture containing 25 mM MES, pH 5. 0, 0. 01 μg rhCathepsin B, 10 μM reaction substrate. The specific activity is >2800 pmol/min/μg.

Form

Lyophilized

Molecular Weight

36.7 kDa (pro-form) and 29 kDa (mature-form)

Storage Conditions

Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.

Notes

For research use only.

Applications Notes

This recombinant protein can be used for WB. For research use only.

Tested Applications

WB

NCBI Accession Number

NP_001899

Preservative

50 mM Tris, 150 mM NaCl, pH 8.0

AA Sequence

Arg 18 - Ile 339

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