PDL1 Recombinant Protein
PDL1 Recombinant Protein
Product Specifications
Product Name Alternative
PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
UniProt
Q9NZQ7
Tag
His Tag
Source
HEK293 cells
Field of Research
Immunology & Inflammation
Purity
>98% as determined by SEC-HPLC and SDS-PAGE.
Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human PDL1 /CD274 Protein at 5μg/mL (100 μl/well), can bind Biotinylated Human PDCD1 Protein, C-Fc, His & AVI Tagged. The EC50 is typically 0. 16-2. 4μg/mL. Measured by its binding ability in a functional ELISA. Immobilized rh PD-L1 / B7-H1 at 2μg/ml (100μl/well) can bind rhPD1/PDCD1 Fc Chimera with a linear range of 0. 05-1μg/ml. Measured by its ability to inhibit antiCD3 antibody induced IL2 secretion in human T lymphocytes. The ED50 for this effect is typically 0. 6-5μg/ml.
Form
Lyophilized
Molecular Weight
26 kDa
Storage Conditions
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Notes
For research use only.
Applications Notes
This recombinant protein can be used for WB. For research use only.
Tested Applications
WB
NCBI Accession Number
NP_054862.1
Preservative
PBS, pH 7.4
AA Sequence
Phe 19 - Arg 238
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