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PDL1 Recombinant Protein

PDL1 Recombinant Protein

Product Specifications

Product Name Alternative

PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1

UniProt

Q9NZQ7

Tag

His Tag

Source

HEK293 cells

Field of Research

Immunology & Inflammation

Purity

>98% as determined by SEC-HPLC and SDS-PAGE.

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Human PDL1 /CD274 Protein at 5μg/mL (100 μl/well), can bind Biotinylated Human PDCD1 Protein, C-Fc, His & AVI Tagged. The EC50 is typically 0. 16-2. 4μg/mL. Measured by its binding ability in a functional ELISA. Immobilized rh PD-L1 / B7-H1 at 2μg/ml (100μl/well) can bind rhPD1/PDCD1 Fc Chimera with a linear range of 0. 05-1μg/ml. Measured by its ability to inhibit anti­CD3 antibody induced IL­2 secretion in human T lymphocytes. The ED50 for this effect is typically 0. 6-5μg/ml.

Form

Lyophilized

Molecular Weight

26 kDa

Storage Conditions

Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.

Notes

For research use only.

Applications Notes

This recombinant protein can be used for WB. For research use only.

Tested Applications

WB

NCBI Accession Number

NP_054862.1

Preservative

PBS, pH 7.4

AA Sequence

Phe 19 - Arg 238

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