TNF alpha Recombinant Protein (HEK293 Expressed)
TNF alpha Recombinant Protein (HEK293 Expressed)
Product Specifications
Product Name Alternative
DIF, TNF-alpha, TNFA, TNFSF2, cachexin, cachectin, TNFα
UniProt
P01375
Tag
Tag free
Source
HEK293 cells
Field of Research
Immunology & Inflammation
Purity
>95% as determined by SDS-PAGE.
Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human TNF-alpha at 5 ug/mL (100 ul/well) can bind Humira with a linear range of 0.04-0.6 ng/mL. The bio-activity was determined by its cytotoxcity assy using L929 Cells with 1ug/ml actinomycin D.
Form
Lyophilized
Molecular Weight
17.4 kDa
Storage Conditions
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Notes
For research use only.
Applications Notes
This recombinant protein can be used for WB. For research use only.
Tested Applications
WB
NCBI Accession Number
P01375
Preservative
PBS, pH 7.4
AA Sequence
Val 77 - Leu 233
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