Human SEMA3A protein

Recombinant Human Semaphorin 3A Protein is produced by mammalian expression system and the target gene encoding Lys26-Phe546 is expressed with a 6His-Flag tag at the N-terminus.

Product Specifications

Product Name Alternative

SEMAD; Semaphorin-3A; Semaphorin III; Sema III

UniProt

Q14563

Reactivity

Human

Source

Human cells

Field of Research

Neuroscience

Endotoxin

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Form

Lyophilized

Storage Conditions

Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Notes

For research use only.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Tested Applications

ELISA, MS, SDS-PAGE, WB

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

AA Sequence

GSGSHHHHHH DYKDDDDKKN NVPRLKLSYK EMLESNNVIT FNGLANSSSY HTFLLDEERS RLYVGAKDHI FSFDLVNIKD FQKIVWPVSY TRRDECKWAG KDILKECANF IKVLKAYNQT HLYACGTGAF HPICTYIEIG HHPEDNIFKL ENSHFENGRG KSPYDPKLLT ASLLIDGELY SGTAADFMGR DFAIFRTLGH HHPIRTEQHD SRWLNDPKFI SAHLISESDN PEDDKVYFFF RENAIDGEHS GKATHARIGQ ICKNDFGGHR SLVNKWTTFL KARLICSVPG PNGIDTHFDE LQDVFLMNFK DPKNPVVYGV FTTSSNIFKG SAVCMYSMSD VRRVFLGPYA HRDGPNYQWV PYQGRVPYPR PGTCPSKTFG GFDSTKDLPD DVITFARSHP AMYNPVFPMN NRPIVIKTDV NYQFTQIVVD RVDAEDGQYD VMFIGTDVGT VLKVVSIPKE TWYDLEEVLL EEMTVFREPT AISAMELSTK QQQLYIGSTA GVAQLPLHRC DIYGKACAEC CLARDPYCAW DGSACSRYF