Human Leptin Receptor protein

Recombinant Human CD295 Protein is produced by mammalian expression system and the target gene encoding Phe22-Asp839 is expressed with a 10His tag at the C-terminus.

Product Specifications

Product Name Alternative

DB; OBR; Leptin receptor; LEP-R; HuB219; OB receptor; OB-R; CD295

UniProt

P48357

Reactivity

Human

Source

Human cells

Field of Research

Metabolism Research

Endotoxin

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Form

Lyophilized

Storage Conditions

Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Notes

For research use only.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Tested Applications

ELISA, MS, SDS-PAGE, WB

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

AA Sequence

FNLSYPITPW RFKLSCMPPN STYDYFLLPA GLSKNTSNSN GHYETAVEPK FNSSGTHFSN LSKTTFHCCF RSEQDRNCSL CADNIEGKTF VSTVNSLVFQ QIDANWNIQC WLKGDLKLFI CYVESLFKNL FRNYNYKVHL LYVLPEVLED SPLVPQKGSF QMVHCNCSVH ECCECLVPVP TAKLNDTLLM CLKITSGGVI FQSPLMSVQP INMVKPDPPL GLHMEITDDG NLKISWSSPP LVPFPLQYQV KYSENSTTVI READKIVSAT SLLVDSILPG SSYEVQVRGK RLDGPGIWSD WSTPRVFTTQ DVIYFPPKIL TSVGSNVSFH CIYKKENKIV PSKEIVWWMN LAEKIPQSQY DVVSDHVSKV TFFNLNETKP RGKFTYDAVY CCNEHECHHR YAELYVIDVN INISCETDGY LTKMTCRWST STIQSLAEST LQLRYHRSSL YCSDIPSIHP ISEPKDCYLQ SDGFYECIFQ PIFLLSGYTM WIRINHSLGS LDSPPTCVLP DSVVKPLPPS SVKAEITINI GLLKISWEKP VFPENNLQFQ IRYGLSGKEV QWKMYEVYDA KSKSVSLPVP DLCAVYAVQV RCKRLDGLGY WSNWSNPAYT VVMDIKVPMR GPEFWRIING DTMKKEKNVT LLWKPLMKND SLCSVQRYVI NHHTSCNGTW SEDVGNHTKF TFLWTEQAHT VTVLAINSIG ASVANFNLTF SWPMSKVNIV QSLSAYPLNS SCVIVSWILS PSDYKLMYFI IEWKNLNEDG EIKWLRISSS VKKYYIHDHF IPIEKYQFSL YPIFMEGVGK PKIINSFTQD DIEKHQSDHH HHHHHHHH