Human USP14 protein

Recombinant human USP14 protein is produced by our E. coli expression system and the target gene encoding Asp91-Gln494 is expressed with a 6His tag at the N-terminus.

Product Specifications

Product Name Alternative

TGT; Ubiquitin carboxyl-terminal hydrolase 14; Deubiquitinating enzyme 14; Ubiquitin thioesterase 14; Ubiquitin-specific-processing protease 14

UniProt

P54578

Reactivity

Human

Source

E. coli

Field of Research

Immunology & Inflammation

Endotoxin

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.

Form

Liquid

Storage Conditions

Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Tested Applications

ELISA, MS, SDS-PAGE, WB

Preservative

0.2 μM filtered solution of 20mM Tris-HCl, 100mM NaCl, 20% Glycerol, pH 8.0.

AA Sequence

MGSSHHHHHH SSGLVPRGSH MDMTEEQLAS AMELPCGLTN LGNTCYMNAT VQCIRSVPEL KDALKRYAGA LRASGEMASA QYITAALRDL FDSMDKTSSS IPPIILLQFL HMAFPQFAEK GEQGQYLQQD ANECWIQMMR VLQQKLEAIE DDSVKETDSS SASAATPSKK KSLIDQFFGV EFETTMKCTE SEEEEVTKGK ENQLQLSCFI NQEVKYLFTG LKLRLQEEIT KQSPTLQRNA LYIKSSKISR LPAYLTIQMV RFFYKEKESV NAKVLKDVKF PLMLDMYELC TPELQEKMVS FRSKFKDLED KKVNQQPNTS DKKSSPQKEV KYEPFSFADD IGSNNCGYYD LQAVLTHQGR SSSSGHYVSW VKRKQDEWIK FDDDKVSIVT PEDILRLSGG GDWHIAYVLL YGPRRVEIME EESEQ