Human UCHL3 protein
Recombinant human UCHL3 protein is produced by our E. coli expression system and the target gene encoding Met1-Ala230 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
Ubiquitin carboxyl-terminal hydrolase isozyme L3; UCH-L3; Ubiquitin thioesterase L3
UniProt
P15374
Reactivity
Human
Source
E. coli
Field of Research
Epigenetics
Endotoxin
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Purity
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Form
Liquid
Storage Conditions
Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Tested Applications
ELISA, MS, SDS-PAGE, WB
Preservative
0.2 μM filtered solution of 50mM Tris-HCl, 150mM NaCl, 1mM DTT, 50% Glycerol, pH 8.0
AA Sequence
MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERARYLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGETSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA LEHHHHHH
Entrez
7347
Available Sizes
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