Human UBE2C protein

Recombinant human UBE2C protein is produced by our E. coli expression system and the target gene encoding Met1-Pro179 is expressed with a 6His, T7 tag at the N-terminus.

Product Specifications

Product Name Alternative

UBCH10; Ubiquitin-conjugating enzyme E2 C; UbcH10; Ubiquitin carrier protein C; Ubiquitin-protein ligase C

UniProt

O00762

Reactivity

Human

Source

E. coli

Field of Research

Epigenetics

Endotoxin

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Form

Liquid

Storage Conditions

Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Notes

For research use only.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Tested Applications

ELISA, MS, SDS-PAGE, WB

Preservative

0.2 μM filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, pH 7.5.

AA Sequence

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSHMASQN RDPAATSVAA ARKGAEPSGG AARGPVGKRL QQELMTLMMS GDKGISAFPE SDNLFKWVGT IHGAAGTVYE DLRYKLSLEF PSGYPYNAPT VKFLTPCYHP NVDTQGNICL DILKEKWSAL YDVRTILLSI QSLLGEPNID SPLNTHAAEL WKNPTAFKKY LQETYSKQVT SQEP