Rabbit TNF protein

Recombinant rabbit TNF protein is produced by our E. coli using rDNA technology. The protein consists of three identical polypeptide chains of 158 amino acids combined to form a compact, bell-shaped homotrimer. The individual subunits have a relative molecular mass each of 17.4 Daltons.

Product Specifications

Product Name Alternative

TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

UniProt

P04924

Reactivity

Rabbit

Source

E. coli

Field of Research

Immunology & Inflammation

Endotoxin

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.

Form

Lyophilized

Storage Conditions

Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Notes

For research use only.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Tested Applications

ELISA, MS, SDS-PAGE, WB

Preservative

0.2 μM filtered solution of 20mM PB, 300mM NaCl, pH 7.4.

AA Sequence

MLRSASRALS DKPLAHVVAN PQVEGQLQWL SQRANALLAN GMKLTDNQLV VPADGLYLIY SQVLFSGQGC RSYVLLTHTV SRFAVSYPNK VNLLSAIKSP CHRETPEEAE PMAWYEPIYL GGVFQLEKGD RLSTEVNQPE YLDLAESGQV YFGIIAL