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Human TNF protein

Recombinant human TNF protein is produced by our E. coli using rDNA technology. The protein consists of three identical polypeptide chains of 158 amino acids combined to form a compact bell-shaped homotrimer. The individual subunits have a relative molecular mass of 17484 Daltons each. TNF-alpha is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.

Product Specifications

Product Name Alternative

TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

UniProt

P01375

Reactivity

Human

Source

E. coli

Field of Research

Immunology & Inflammation

Endotoxin

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.

Form

Lyophilized

Storage Conditions

Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Notes

For research use only.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Tested Applications

ELISA, MS, SDS-PAGE, WB

Preservative

0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.0.

AA Sequence

MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL

Entrez

7124

Available Sizes

Frequently Asked Questions

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