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Human SMAD1 protein

Recombinant human SMAD1 protein is produced by our E. coli expression system and the target gene encoding Met1-Ser465 is expressed with a GST tag at the N-terminus.

Product Specifications

Product Name Alternative

BSP1; MADH1; MADR1; Mothers against decapentaplegic homolog 1; MAD homolog 1; Mothers against DPP homolog 1; JV4-1; Mad-related protein 1; SMAD family member 1; SMAD 1; Smad1; hSMAD1; Transforming growth factor-beta-signaling protein 1; BSP-1

UniProt

Q15797

Reactivity

Human

Source

E. coli

Field of Research

Neuroscience

Endotoxin

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Form

Lyophilized

Storage Conditions

Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Notes

For research use only.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Tested Applications

ELISA, MS, SDS-PAGE, WB

Preservative

0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.

AA Sequence

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSHMNV TSLFSFTSPA VKRLLGWKQG DEEEKWAEKA VDALVKKLKK KKGAMEELEK ALSCPGQPSN CVTIPRSLDG RLQVSHRKGL PHVIYCRVWR WPDLQSHHEL KPLECCEFPF GSKQKEVCIN PYHYKRVESP VLPPVLVPRH SEYNPQHSLL AQFRNLGQNE PHMPLNATFP DSFQQPNSHP FPHSPNSSYP NSPGSSSSTY PHSPTSSDPG SPFQMPADTP PPAYLPPEDP MTQDGSQPMD TNMMAPPLPS EINRGDVQAV AYEEPKHWCS IVYYELNNRV GEAFHASSTS VLVDGFTDPS NNKNRFCLGL LSNVNRNSTI ENTRRHIGKG VHLYYVGGEV YAECLSDSSI FVQSRNCNYH HGFHPTTVCK IPSGCSLKIF NNQEFAQLLA QSVNHGFETV YELTKMCTIR MSFVKGWGAE YHRQDVTSTP CWIEIHLHGP LQWLDKVLTQ MGSPHNPISS VS

Entrez

4086

Available Sizes

Frequently Asked Questions

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