Human SIAE protein

Recombinant human SIAE protein is produced by our mammalian expression system and the target gene encoding Ile24-Lys523 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

YSG2; Sialate O-acetylesterase; H-Lse; Sialic acid-specific 9-O-acetylesterase

UniProt

Q9HAT2

Reactivity

Human

Source

Human cells

Field of Research

Epigenetics

Endotoxin

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Form

Liquid

Storage Conditions

Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Tested Applications

ELISA, MS, SDS-PAGE, WB

Preservative

0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 7.5.

AA Sequence

IGFRFASYIN NDMVLQKEPA GAVIWGFGTP GATVTVTLRQ GQETIMKKVT SVKAHSDTWM VVLDPMKPGG PFEVMAQQTL EKINFTLRVH DVLFGDVWLC SGQSNMQMTV LQIFNATREL SNTAAYQSVR ILSVSPIQAE QELEDLVAVD LQWSKPTSEN LGHGYFKYMS AVCWLFGRHL YDTLQYPIGL IASSWGGTPI EAWSSGRSLK ACGVPKQGSI PYDSVTGPSK HSVLWNAMIH PLCNMTLKGV VWYQGESNIN YNTDLYNCTF PALIEDWRET FHRGSQGQTE RFFPFGLVQL SSDLSKKSSD DGFPQIRWHQ TADFGYVPNP KMPNTFMAVA MDLCDRDSPF GSIHPRDKQT VAYRLHLGAR ALAYGEKNLT FEGPLPEKIE LLAHKGLLNL TYYQQIQVQK KDNKIFEISC CSDHRCKWLP ASMNTVSTQS LTLAIDSCHG TVVALRYAWT TWPCEYKQCP LYHPSSALPA PPFIAFITDQ GPGHQSNVAK VDHHHHHH