Human PRKCE protein
Recombinant human PRKCE protein is produced by our E. coli expression system and the target gene encoding Gln580-Pro737 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
PKCE; Protein kinase C epsilon type; nPKC-epsilon
UniProt
Q02156
Reactivity
Human
Source
E. coli
Field of Research
Signal Transduction
Endotoxin
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Purity
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Form
Liquid
Storage Conditions
Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Tested Applications
ELISA, MS, SDS-PAGE, WB
Preservative
0.2 μM filtered solution of 20mM PB, 150mM NaCl, 1mM DTT, pH 7.4.
AA Sequence
MQELEYGPSV DWWALGVLMY EMMAGQPPFE ADNEDDLFES ILHDDVLYPV WLSKEAVSIL KAFMTKNPHK RLGCVASQNG EDAIKQHPFF KEIDWVLLEQ KKIKPPFKPR IKTKRDVNNF DQDFTREEPV LTLVDEAIVK QINQEEFKGF SYFGEDLMPL EHHHHHH
Entrez
5581
Available Sizes
Frequently Asked Questions
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