Human OTUB2 protein

Recombinant human OTUB2 protein is produced by our E.coli expression system and the target gene encoding Met1-His234 is expressed with a GST tag at the N-terminus.

Product Specifications

Product Name Alternative

C14orf137; OTB2; OTU2; Ubiquitin thioesterase OTUB2; Deubiquitinating enzyme OTUB2; OTU domain-containing ubiquitin aldehyde-binding protein 2; Otubain-2; Ubiquitin-specific-processing protease OTUB2

UniProt

Q96DC9

Reactivity

Human

Source

E. coli

Endotoxin

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Form

Liquid

Storage Conditions

Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Tested Applications

ELISA, MS, SDS-PAGE, WB

Preservative

0.2 μM filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, pH 7.5

AA Sequence

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSHMSE TSFNLISEKC DILSILRDHP ENRIYRRKIE ELSKRFTAIR KTKGDGNCFY RALGYSYLES LLGKSREIFK FKERVLQTPN DLLAAGFEEH KFRNFFNAFY SVVELVEKDG SVSSLLKVFN DQSASDHIVQ FLRLLTSAFI RNRADFFRHF IDEEMDIKDF CTHEVEPMAT ECDHIQITAL SQALSIALQV EYVDEMDTAL NHHVFPEAAT PSVYLLYKTS HYNILYAADK H