Human NOL3 protein
Recombinant human NOL3 protein is produced by our E.coli expression system and the target gene encoding Met1-Ser208 is expressed with a GST tag at the N-terminus.
Product Specifications
Product Name Alternative
Myp; NOL3; Nop30; Nucleolar protein 3; apoptosis repressor ARC; apoptosis repressor with CARD; apoptosis repressor with caspase recruitment domain (CARD) ; muscle-enriched cytoplasmic protein; nucleolar protein of 30 kDa
UniProt
O60936
Reactivity
Human
Source
E. coli
Field of Research
Cell Biology
Endotoxin
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Purity
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Form
Lyophilized
Storage Conditions
Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Notes
For research use only.
Applications Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Tested Applications
ELISA, MS, SDS-PAGE, WB
Preservative
0.2 μM filtered solution of 20mM Tris-HCl, 1mM DTT, 150mM NaCl, pH 8.0.
AA Sequence
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMGNA QERPSETIDR ERKRLVETLQ ADSGLLLDAL LARGVLTGPE YEALDALPDA ERRVRRLLLL VQGKGEAACQ ELLRCAQRTA GAPDPAWDWQ HVGPGYRDRS YDPPCPGHWT PEAPGSGTTC PGLPRASDPD EAGGPEGSEA VQSGTPEEPE PELEAEASKE AEPEPEPEPE LEPEAEAEPE PELEPEPDPE PEPDFEERDE SEDS
Entrez
8996
Available Sizes
Frequently Asked Questions
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