Human NEDD8 protein
Recombinant human NEDD8 protein is produced by our E. coli expression system and the target gene encoding Met1-Gly76 is expressed with a 6His, SUMO tag at the N-terminus.
Product Specifications
Product Name Alternative
NEDD8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8
UniProt
Q15843
Reactivity
Human
Source
E. coli
Field of Research
Epigenetics
Endotoxin
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Form
Lyophilized
Storage Conditions
Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Notes
For research use only.
Applications Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Tested Applications
ELISA, MS, SDS-PAGE, WB
Preservative
0.2 μM filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose, pH 7.4.
AA Sequence
MGHHHHHHGS LQDSEVNQEA KPEVKPEVKP ETHINLKVSD GSSEIFFKIK KTTPLRRLME AFAKRQGKEM DSLRFLYDGI RIQADQAPED LDMEDNDIIE AHREQIGGML IKVKTLTGKE IEIDIEPTDK VERIKERVEE KEGIPPQQQR LIYSGKQMND EKTAADYKIL GGSVLHLVLA LRGG
Entrez
4738
Available Sizes
Frequently Asked Questions
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