Human MMP9 protein

Recombinant human MMP9 protein is produced by our mammalian expression system and the target gene encoding Ala19-Asp707 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

CLG4B; Matrix metalloproteinase-9; MMP-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB

UniProt

P14780

Reactivity

Human

Source

Human cells

Field of Research

Cancer Biology

Endotoxin

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Form

Liquid

Storage Conditions

Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Tested Applications

ELISA, MS, SDS-PAGE, WB

Preservative

0.2 μM filtered solution of 20mM Tris-HCl, 2mM CaCl2, 150mM NaCl, 0.05% Brij35, pH 7.5.

AA Sequence

AAPRQRQSTL VLFPGDLRTN LTDRQLAEEY LYRYGYTRVA EMRGESKSLG PALLLLQKQL SLPETGELDS ATLKAMRTPR CGVPDLGRFQ TFEGDLKWHH HNITYWIQNY SEDLPRAVID DAFARAFALW SAVTPLTFTR VYSRDADIVI QFGVAEHGDG YPFDGKDGLL AHAFPPGPGI QGDAHFDDDE LWSLGKGVVV PTRFGNADGA ACHFPFIFEG RSYSACTTDG RSDGLPWCST TANYDTDDRF GFCPSERLYT RDGNADGKPC QFPFIFQGQS YSACTTDGRS DGYRWCATTA NYDRDKLFGF CPTRADSTVM GGNSAGELCV FPFTFLGKEY STCTSEGRGD GRLWCATTSN FDSDKKWGFC PDQGYSLFLV AAHEFGHALG LDHSSVPEAL MYPMYRFTEG PPLHKDDVNG IRHLYGPRPE PEPRPPTTTT PQPTAPPTVC PTGPPTVHPS ERPTAGPTGP PSAGPTGPPT AGPSTATTVP LSPVDDACNV NIFDAIAEIG NQLYLFKDGK YWRFSEGRGS RPQGPFLIAD KWPALPRKLD SVFEEPLSKK LFFFSGRQVW VYTGASVLGP RRLDKLGLGA DVAQVTGALR SGRGKMLLFS GRRLWRFDVK AQMVDPRSAS EVDRMFPGVP LDTHDVFQYR EKAYFCQDRF YWRVSSRSEL NQVDQVGYVT YDILQCPEDV DHHHHHH