Human LEP protein
Recombinant human LEP protein is produced by our E. coli expression system and the target gene encoding Val22-Cys167 is expressed.
Product Specifications
Product Name Alternative
OB; OBS; Leptin; Obese protein; Obesity factor
UniProt
P41159
Reactivity
Human
Source
E. coli
Field of Research
Epigenetics
Endotoxin
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Purity
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Form
Lyophilized
Storage Conditions
Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Notes
For research use only.
Applications Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Tested Applications
ELISA, MS, SDS-PAGE, WB
Preservative
0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
AA Sequence
VPIQKVQDDT KTLIKTIVTR INDISHTQSV SSKQKVTGLD FIPGLHPILT LSKMDQTLAV YQQILTSMPS RNVIQISNDL ENLRDLLHVL AFSKSCHLPW ASGLETLDSL GGVLEASGYS TEVVALSRLQ GSLQDMLWQL DLSPGC
Entrez
3952
Available Sizes
Frequently Asked Questions
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