Human KARS protein
Recombinant human KARS protein is produced by our mammalian expression system and the target gene encoding Ala2-Val597 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
KIAA0070; Lysine--tRNA ligase; Lysyl-tRNA synthetase; LysRS
UniProt
Q15046
Reactivity
Human
Source
Human cells
Field of Research
Neuroscience
Endotoxin
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Form
Liquid
Storage Conditions
Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Tested Applications
ELISA, MS, SDS-PAGE, WB
Preservative
0.2 μM filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 20% Glycerol, pH 8.0.
AA Sequence
AAVQAAEVKV DGSEPKLSKN ELKRRLKAEK KVAEKEAKQK ELSEKQLSQA TAAATNHTTD NGVGPEEESV DPNQYYKIRS QAIHQLKVNG EDPYPHKFHV DISLTDFIQK YSHLQPGDHL TDITLKVAGR IHAKRASGGK LIFYDLRGEG VKLQVMANSR NYKSEEEFIH INNKLRRGDI IGVQGNPGKT KKGELSIIPY EITLLSPCLH MLPHLHFGLK DKETRYRQRY LDLILNDFVR QKFIIRSKII TYIRSFLDEL GFLEIETPMM NIIPGGAVAK PFITYHNELD MNLYMRIAPE LYHKMLVVGG IDRVYEIGRQ FRNEGIDLTH NPEFTTCEFY MAYADYHDLM EITEKMVSGM VKHITGSYKV TYHPDGPEGQ AYDVDFTPPF RRINMVEELE KALGMKLPET NLFETEETRK ILDDICVAKA VECPPPRTTA RLLDKLVGEF LEVTCINPTF ICDHPQIMSP LAKWHRSKEG LTERFELFVM KKEICNAYTE LNDPMRQRQL FEEQAKAKAA GDDEAMFIDE NFCTALEYGL PPTAGWGMGI DRVAMFLTDS NNIKEVLLFP AMKPEDKKEN VATTDTLEST TVGTSVVDHH HHHH
Entrez
3735
Available Sizes
Frequently Asked Questions
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