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Human HMGB3 protein

Recombinant human HMGB3 protein is produced by our mammalian expression system and the target gene encoding Met1-Glu200 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

HMG2A; HMG4; High mobility group protein B3; High mobility group protein 2a; HMG-2a; High mobility group protein 4; HMG-4

UniProt

O15347

Reactivity

Human

Source

Human cells

Field of Research

Immunology & Inflammation

Endotoxin

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Form

Lyophilized

Storage Conditions

Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Notes

For research use only.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Tested Applications

ELISA, MS, SDS-PAGE, WB

Preservative

0.2 μM filtered solution of PBS, pH 7.4.

AA Sequence

MAKGDPKKPK GKMSAYAFFV QTCREEHKKK NPEVPVNFAE FSKKCSERWK TMSGKEKSKF DEMAKADKVR YDREMKDYGP AKGGKKKKDP NAPKRPPSGF FLFCSEFRPK IKSTNPGISI GDVAKKLGEM WNNLNDSEKQ PYITKAAKLK EKYEKDVADY KSKGKFDGAK GPAKVARKKV EEEDEEEEEE EEEEEEEEDE VDHHHHHH

Entrez

3149

Available Sizes

Frequently Asked Questions

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