Human HMGB1 protein

Recombinant human HMGB1 protein is produced by our E. coli expression system and the target gene encoding Pro92-Val176 is expressed with a MARI tag at the N-terminus.

Product Specifications

Product Name Alternative

HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1

UniProt

P09429

Reactivity

Human

Source

E. coli

Field of Research

Autophagic, Cancer, DNA Damage, Epigenetics, Immunology, Infectious Diseases, Neuroscience, Signaling Pathways

Endotoxin

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Form

Lyophilized

Storage Conditions

Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Notes

For research use only.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.