Human HMGB1 protein
Recombinant human HMGB1 protein is produced by our E. coli expression system and the target gene encoding Pro92-Val176 is expressed with a MARI tag at the N-terminus.
Product Specifications
Product Name Alternative
HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1
UniProt
P09429
Reactivity
Human
Source
E. coli
Field of Research
Autophagic, Cancer, DNA Damage, Epigenetics, Immunology, Infectious Diseases, Neuroscience, Signaling Pathways
Endotoxin
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Form
Lyophilized
Storage Conditions
Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Notes
For research use only.
Applications Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Tested Applications
ELISA, MS, SDS-PAGE, WB
Preservative
0.2 μM filtered solution of 50mM HEPES, 500mM NaCl, 0.6mM DTT, pH 7.9.
AA Sequence
MARIDPNAPK RPPSAFFLFC SEYRPKIKGE HPGLSIGDVA KKLGEMWNNT AADDKQPYEK KAAKLKEKYE KDIAAYRAKG KPDAAKKGVV
Entrez
3146
Available Sizes
Frequently Asked Questions
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