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Human GCA protein

Recombinant human GCA protein is produced by our E.coli expression system and the target gene encoding Met1-Ile217 is expressed with a GST tag at the N-terminus.

Product Specifications

Product Name Alternative

GCL; Grancalcin

UniProt

P28676

Reactivity

Human

Source

E. coli

Endotoxin

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.

Form

Lyophilized

Storage Conditions

Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Notes

For research use only.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Tested Applications

ELISA, MS, SDS-PAGE, WB

Preservative

0.2 μM filtered solution of 20mM Tris-HCl, 500mM NaCl, pH 8.5.

AA Sequence

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMAYP GYGGGFGNFS IQVPGMQMGQ PVPETGPAIL LDGYSGPAYS DTYSSAGDSV YTYFSAVAGQ DGEVDAEELQ RCLTQSGING TYSPFSLETC RIMIAMLDRD HTGKMGFNAF KELWAALNAW KENFMTVDQD GSGTVEHHEL RQAIGLMGYR LSPQTLTTIV KRYSKNGRIF FDDYVACCVK LRALTDFFRK RDHLQQGSAN FIYDDFLQGT MAI

Entrez

25801

Available Sizes

Frequently Asked Questions

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