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Human GADD45A protein

Recombinant human GADD45A protein is produced by our E. coli expression system and the target gene encoding Met1-Arg165 is expressed with a 6His tag at the N-terminus.

Product Specifications

Product Name Alternative

DDIT1; GADD45; Growth arrest and DNA damage-inducible protein GADD45 alpha; DNA damage-inducible transcript 1 protein; DDIT-1

UniProt

P24522

Reactivity

Human

Source

E. coli

Field of Research

Cancer Biology

Endotoxin

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Form

Lyophilized

Storage Conditions

Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Notes

For research use only.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Tested Applications

ELISA, MS, SDS-PAGE, WB

Preservative

0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

AA Sequence

MGSSHHHHHH SSGLVPRGSH MTLEEFSAGE QKTERMDKVG DALEEVLSKA LSQRTITVGV YEAAKLLNVD PDNVVLCLLA ADEDDDRDVA LQIHFTLIQA FCCENDINIL RVSNPGRLAE LLLLETDAGP AASEGAEQPP DLHCVLVTNP HSSQWKDPAL SQLICFCRES RYMDQWVPVI NLPER

Entrez

1647

Available Sizes

Frequently Asked Questions

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