Human EDF1 protein

Recombinant human EDF1 protein is produced by our E. coli expression system and the target gene encoding Ala2-Lys148 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

Endothelial differentiation-related factor 1; EDF-1; Multiprotein-bridging factor 1; MBF1

UniProt

O60869

Reactivity

Human

Source

E. coli

Field of Research

Stem Cell & Developmental Biology

Endotoxin

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Form

Lyophilized

Storage Conditions

Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Notes

For research use only.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Tested Applications

ELISA, MS, SDS-PAGE, WB

Preservative

0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.

AA Sequence

AESDWDTVTV LRKKGPTAAQ AKSKQAILAA QRRGEDVETS KKWAAGQNKQ HSITKNTAKL DRETEELHHD RVTLEVGKVI QQGRQSKGLT QKDLATKINE KPQVIADYES GRAIPNNQVL GKIERAIGLK LRGKDIGKPI EKGPRAKLEH HHHHH