Human DNAJB1 protein

Recombinant human DNAJB1 protein is produced by our E. coli expression system and the target gene encoding Gly2-Ile340 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

DNAJ1; HDJ1; HSPF1; DnaJ homolog subfamily B member 1; DnaJ protein homolog 1; Heat shock 40 kDa protein 1; HSP40; Heat shock protein 40; Human DnaJ protein 1; hDj-1

UniProt

P25685

Reactivity

Human

Source

E. coli

Field of Research

Epigenetics

Endotoxin

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Form

Lyophilized

Storage Conditions

Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Notes

For research use only.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Tested Applications

ELISA, MS, SDS-PAGE, WB

Preservative

0.2 μM filtered solution of 20mM PB, 150mM NaCl, 1mM EDTA, pH 7.4.

AA Sequence

GKDYYQTLGL ARGASDEEIK RAYRRQALRY HPDKNKEPGA EEKFKEIAEA YDVLSDPRKR EIFDRYGEEG LKGSGPSGGS GGGANGTSFS YTFHGDPHAM FAEFFGGRNP FDTFFGQRNG EEGMDIDDPF SGFPMGMGGF TNVNFGRSRS AQEPARKKQD PPVTHDLRVS LEEIYSGCTK KMKISHKRLN PDGKSIRNED KILTIEVKKG WKEGTKITFP KEGDQTSNNI PADIVFVLKD KPHNIFKRDG SDVIYPARIS LREALCGCTV NVPTLDGRTI PVVFKDVIRP GMRRKVPGEG LPLPKTPEKR GDLIIEFEVI FPERIPQTSR TVLEQVLPIL EHHHHHH