Human DCK protein

Recombinant human DCK protein is produced by our E. coli expression system and the target gene encoding Met1-Leu260 is expressed with a 6His, T7 tag at the N-terminus.

Product Specifications

Product Name Alternative

Deoxycytidine kinase; dCK

UniProt

P27707

Reactivity

Human

Source

E. coli

Field of Research

Signal Transduction

Endotoxin

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Form

Liquid

Storage Conditions

Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Tested Applications

ELISA, MS, SDS-PAGE, WB

Preservative

0.2 μM filtered solution of 20mM Tris-HCl, pH 7.5.

AA Sequence

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSMATPPK RSCPSFSASS EGTRIKKISI EGNIAAGKST FVNILKQLCE DWEVVPEPVA RWCNVQSTQD EFEELTMSQK NGGNVLQMMY EKPERWSFTF QTYACLSRIR AQLASLNGKL KDAEKPVLFF ERSVYSDRYI FASNLYESEC MNETEWTIYQ DWHDWMNNQF GQSLELDGII YLQATPETCL HRIYLRGRNE EQGIPLEYLE KLHYKHESWL LHRTLKTNFD YLQEVPILTL DVNEDFKDKY ESLVEKVKEF LSTL