Human Collagen IX protein

Recombinant human Collagen IX protein is produced by our mammalian expression system and the target gene encoding Ala24-Pro328 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

Collagen alpha-1 (IX) chain

UniProt

P20849

Reactivity

Human

Source

Human cells

Field of Research

Musculoskeletal & Connective Tissue Research

Endotoxin

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Form

Lyophilized

Storage Conditions

Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Notes

For research use only.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Tested Applications

ELISA, MS, SDS-PAGE, WB

Preservative

0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

AA Sequence

AVKRRPRFPV NSNSNGGNEL CPKIRIGQDD LPGFDLISQF QVDKAASRRA IQRVVGSATL QVAYKLGNNV DFRIPTRNLY PSGLPEEYSF LTTFRMTGST LKKNWNIWQI QDSSGKEQVG IKINGQTQSV VFSYKGLDGS LQTAAFSNLS SLFDSQWHKI MIGVERSSAT LFVDCNRIES LPIKPRGPID IDGFAVLGKL ADNPQVSVPF ELQWMLIHCD PLRPRRETCH ELPARITPSQ TTDERGPPGE QGPPGPPGPP GVPGIDGIDG DRGPKGPPGP PGPAGEPGKP GAPGKPGTPG ADTSPVDHHH HHH