Human Collagen VIII protein
Recombinant human Collagen VIII protein is produced by our Mammalian expression system and the target gene encoding Gly28-Met744 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
C3orf7; Collagen alpha-1 (VIII) chain; Endothelial collagen
UniProt
P27658
Reactivity
Human
Source
Human cells
Field of Research
Musculoskeletal & Connective Tissue Research
Endotoxin
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Purity
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Form
Lyophilized
Storage Conditions
Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Notes
For research use only.
Applications Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Tested Applications
ELISA, MS, SDS-PAGE, WB
Preservative
0.2 μM filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.
AA Sequence
GAYYGIKPLP PQIPPQMPPQ IPQYQPLGQQ VPHMPLAKDG LAMGKEMPHL QYGKEYPHLP QYMKEIQPAP RMGKEAVPKK GKEIPLASLR GEQGPRGEPG PRGPPGPPGL PGHGIPGIKG KPGPQGYPGV GKPGMPGMPG KPGAMGMPGA KGEIGQKGEI GPMGIPGPQG PPGPHGLPGI GKPGGPGLPG QPGPKGDRGP KGLPGPQGLR GPKGDKGFGM PGAPGVKGPP GMHGPPGPVG LPGVGKPGVT GFPGPQGPLG KPGAPGEPGP QGPIGVPGVQ GPPGIPGIGK PGQDGIPGQP GFPGGKGEQG LPGLPGPPGL PGIGKPGFPG PKGDRGMGGV PGALGPRGEK GPIGAPGIGG PPGEPGLPGI PGPMGPPGAI GFPGPKGEGG IVGPQGPPGP KGEPGLQGFP GKPGFLGEVG PPGMRGLPGP IGPKGEAGQK GVPGLPGVPG LLGPKGEPGI PGDQGLQGPP GIPGIGGPSG PIGPPGIPGP KGEPGLPGPP GFPGIGKPGV AGLHGPPGKP GALGPQGQPG LPGPPGPPGP PGPPAVMPPT PPPQGEYLPD MGLGIDGVKP PHAYGAKKGK NGGPAYEMPA FTAELTAPFP PVGAPVKFNK LLYNGRQNYN PQTGIFTCEV PGVYYFAYHV HCKGGNVWVA LFKNNEPVMY TYDEYKKGFL DQASGSAVLL LRPGDRVFLQ MPSEQAAGLY AGQYVHSSFS GYLLYPMVDH HHHHH
Entrez
1295
Available Sizes
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