Human CFB protein

Recombinant human CFB protein is produced by our mammalian expression system and the target gene encoding Thr26-Leu764 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

BF; BFD; Complement factor B; C3/C5 convertase; Glycine-rich beta glycoprotein; GBG; PBF2; Properdin factor B

UniProt

P00751

Reactivity

Human

Source

Human cells

Field of Research

Immunology & Inflammation

Endotoxin

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Form

Liquid

Storage Conditions

Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Tested Applications

ELISA, MS, SDS-PAGE, WB

Preservative

0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.

AA Sequence

TPWSLAWPQG SCSLEGVEIK GGSFRLLQEG QALEYVCPSG FYPYPVQTRT CRSTGSWSTL KTQDQKTVRK AECRAIHCPR PHDFENGEYW PRSPYYNVSD EISFHCYDGY TLRGSANRTC QVNGRWSGQT AICDNGAGYC SNPGIPIGTR KVGSQYRLED SVTYHCSRGL TLRGSQRRTC QEGGSWSGTE PSCQDSFMYD TPQEVAEAFL SSLTETIEGV DAEDGHGPGE QQKRKIVLDP SGSMNIYLVL DGSDSIGASN FTGAKKCLVN LIEKVASYGV KPRYGLVTYA TYPKIWVKVS EADSSNADWV TKQLNEINYE DHKLKSGTNT KKALQAVYSM MSWPDDVPPE GWNRTRHVII LMTDGLHNMG GDPITVIDEI RDLLYIGKDR KNPREDYLDV YVFGVGPLVN QVNINALASK KDNEQHVFKV KDMENLEDVF YQMIDESQSL SLCGMVWEHR KGTDYHKQPW QAKISVIRPS KGHESCMGAV VSEYFVLTAA HCFTVDDKEH SIKVSVGGEK RDLEIEVVLF HPNYNINGKK EAGIPEFYDY DVALIKLKNK LKYGQTIRPI CLPCTEGTTR ALRLPPTTTC QQQKEELLPA QDIKALFVSE EEKKLTRKEV YIKNGDKKGS CERDAQYAPG YDKVKDISEV VTPRFLCTGG VSPYADPNTC RGDSGGPLIV HKRSRFIQVG VISWGVVDVC KNQKRQKQVP AHARDFHINL FQVLPWLKEK LQDEDLGFLV DHHHHHH