Human CA4 protein

Recombinant human CA4 protein is produced by our E.coli expression system and the target gene encoding Ala19-Lys283 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

Carbonic anhydrase 4; Carbonate dehydratase IV; Carbonic anhydrase IV; CA-IV

UniProt

P22748

Reactivity

Human

Source

E. coli

Field of Research

Epigenetics

Endotoxin

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.

Form

Liquid

Storage Conditions

Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Tested Applications

ELISA, MS, SDS-PAGE, WB

Preservative

0.2 μM filtered solution of 20mM Tris-HCl, 100mM NaCl, pH 8.5.

AA Sequence

MAESHWCYEV QAESSNYPCL VPVKWGGNCQ KDRQSPINIV TTKAKVDKKL GRFFFSGYDK KQTWTVQNNG HSVMMLLENK ASISGGGLPA PYQAKQLHLH WSDLPYKGSE HSLDGEHFAM EMHIVHEKEK GTSRNVKEAQ DPEDEIAVLA FLVEAGTQVN EGFQPLVEAL SNIPKPEMST TMAESSLLDL LPKEEKLRHY FRYLGSLTTP TCDEKVVWTV FREPIQLHRE QILAFSQKLY YDKEQTVSMK DNVRPLQQLG QRTVIKLEHH HHHH