Human BLK protein
Recombinant human BLK protein is produced by our E. coli expression system and the target gene encoding Gly2-Pro505 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
Tyrosine-protein kinase Blk; B lymphocyte kinase; p55-Blk
UniProt
P51451
Reactivity
Human
Source
E. coli
Field of Research
Cell Biology
Endotoxin
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Form
Liquid
Storage Conditions
Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Tested Applications
ELISA, MS, SDS-PAGE, WB
Preservative
0.2 μM filtered solution of 20mM Tris, 500mM NaCl, 1mM DTT, pH 7.4.
AA Sequence
GLVSSKKPDK EKPIKEKDKG QWSPLKVSAQ DKDAPPLPPL VVFNHLTPPP PDEHLDEDKH FVVALYDYTA MNDRDLQMLK GEKLQVLKGT GDWWLARSLV TGREGYVPSN FVARVESLEM ERWFFRSQGR KEAERQLLAP INKAGSFLIR ESETNKGAFS LSVKDVTTQG ELIKHYKIRC LDEGGYYISP RITFPSLQAL VQHYSKKGDG LCQRLTLPCV RPAPQNPWAQ DEWEIPRQSL RLVRKLGSGQ FGEVWMGYYK NNMKVAIKTL KEGTMSPEAF LGEANVMKAL QHERLVRLYA VVTKEPIYIV TEYMARGCLL DFLKTDEGSR LSLPRLIDMS AQIAEGMAYI ERMNSIHRDL RAANILVSEA LCCKIADFGL ARIIDSEYTA QEGAKFPIKW TAPEAIHFGV FTIKADVWSF GVLLMEVVTY GRVPYPGMSN PEVIRNLERG YRMPRPDTCP PELYRGVIAE CWRSRPEERP TFEFLQSVLE DFYTATERQY ELQPLEHHHH HH
Entrez
640
Available Sizes
Frequently Asked Questions
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