Human BDNF protein

Recombinant human BDNF protein is produced by our E. coli. It is a single non-glycosylated polypeptide chain containing 119 amino acids. Bioactive BDNF is a non-covalently linked homodimer with a molecular mass of 27 kD.

Product Specifications

Product Name Alternative

Brain-derived neurotrophic factor; BDNF; Abrineurin

UniProt

P23560

Reactivity

Human

Source

E. coli

Field of Research

Neuroscience

Endotoxin

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.

Form

Lyophilized

Storage Conditions

Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Notes

For research use only.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.