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Human ATG3 protein

Recombinant human ATG3 protein is produced by our E. coli expression system and the target gene encoding Met1-His287 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

APG3; APG3L; Ubiquitin-like-conjugating enzyme ATG3; Autophagy-related protein 3; APG3-like; hApg3; Protein PC3-96

UniProt

Q9NT62

Reactivity

Human

Source

E. coli

Field of Research

Cell Biology

Endotoxin

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Form

Liquid

Storage Conditions

Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Tested Applications

ELISA, MS, SDS-PAGE, WB

Preservative

0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.0.

AA Sequence

MASMTGGQQM GRGSMQNVIN TVKGKALEVA EYLTPVLKES KFKETGVITP EEFVAAGDHL VHHCPTWQWA TGEELKVKAY LPTGKQFLVT KNVPCYKRCK QMEYSDELEA IIEEDDGDGG WVDTYHNTGI TGITEAVKEI TLENKDNIRL QDCSALCEEE EDEDEGEAAD MEEYEESGLL ETDEATLDTR KIVEACKAKT DAGGEDAILQ TRTYDLYITY DKYYQTPRLW LFGYDEQRQP LTVEHMYEDI SQDHVKKTVT IENHPHLPPP PMCSVHPCRH AEVMKKIIET VAEGGGELGV HLEHHHHHH

Entrez

64422

Available Sizes

Frequently Asked Questions

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