Human ABCB5 protein

Recombinant human ABCB5 protein is produced by our E. coli expression system and the target gene encoding Ile141-Val247 is expressed with a Trx tag at the N-terminus.

Product Specifications

Product Name Alternative

ATP-binding cassette sub-family B member 5; ABCB5 P-gp; P-glycoprotein ABCB5

UniProt

Q2M3G0

Reactivity

Human

Source

E. coli

Field of Research

Stem Cell & Developmental Biology

Endotoxin

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Form

Lyophilized

Storage Conditions

Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Notes

For research use only.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Tested Applications

ELISA, MS, SDS-PAGE, WB

Preservative

0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

AA Sequence

MSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL KEFLDANLAG SGSGHMHHHH HHSSGLVPRG SGMKETAAAK FERQHMDSPD LGTDDDDKAM AIRSADLIVT LKDGMLAEKG AHAELMAKRG LYYSLVMSQD IKKADEQMES MTYSTERKTN SLPLHSVKSI KSDFIDKAEE STQSKEISLP EVSLLKILKL NKPEWPFV