Anti-Atg12 Antibody

Rabbit Polyclonal Antibody specific to Atg12

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Autophagy-related protein 12, APG12-like

Gene Name

ATG12

NCBI Gene ID

UniProt

O94817

Cellular Locus

Cytoplasm, Preautophagosomal structure membrane

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide corresponding to amino acids at the N-terminus of human Atg12.

Target Antigen

Ubiquitin-like protein ATG12

Target

Atg12

Clonality

Polyclonal

Type

Antibody

Applications

WB, ICC/IF

Field of Research

Autophagy

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. {PubMed:12207896, PubMed:17999726, PubMed:19074260, PubMed:19164948, PubMed:23202584}.; (Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection (PubMed:17709747). Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for the initiation of HCV replication, but not required once infection is established (PubMed:19666601). {PubMed:17709747, PubMed:19666601}.

Additionnal Information

Immunoblotting: use at 1-2ug/mL. Bands of ~48-55kDa, corresponding to Atg12-Atg5 complex, are detected. Note: the predicted molecular weight of Atg12 is ~15kDa. Immunofluorescence: use at 10ug/mL. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human Atg12.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Concentration: 1mg/ml

Buffer pH

pH 7.4

Target Background

Autophagy is a catabolic process that results in the degradation of bulk cytoplasmic contents within autophagosomes and lysosomes. Atg12 is an ubiquitin-like protein involved in autophagy vesicle formation. Conjugation with Atg5 through a ubiquitin-like conjugating system involving also Atg7 as an E1-like activating enzyme and Atg10 as an E2-like conjugating enzyme, is essential for its function. The Atg12-Atg5 conjugate acts as an E3-like enzyme which is required for lipidation of Atg8 family proteins and their association to vesicle membranes. The Atg12-Atg5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS.