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Human CA5B protein

Recombinant human CA5B protein is produced by our E.coli expression system and the target gene encoding Cys34-Pro317 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

Carbonic anhydrase 5B, mitochondrial; Carbonate dehydratase VB; Carbonic anhydrase VB; CA-VB

UniProt

Q9Y2D0

Reactivity

Human

Source

E. coli

Endotoxin

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.

Form

Liquid

Storage Conditions

Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Tested Applications

ELISA, MS, SDS-PAGE, WB

Preservative

0.2 μM filtered solution of 20mM Tris-HCl, 100mM NaCl, pH 8.0.

AA Sequence

MCSLYTCTYK TRNRALHPLW ESVDLVPGGD RQSPINIRWR DSVYDPGLKP LTISYDPATC LHVWNNGYSF LVEFEDSTDK SVIKGGPLEH NYRLKQFHFH WGAIDAWGSE HTVDSKCFPA ELHLVHWNAV RFENFEDAAL EENGLAVIGV FLKLGKHHKE LQKLVDTLPS IKHKDALVEF GSFDPSCLMP TCPDYWTYSG SLTTPPLSES VTWIIKKQPV EVDHDQLEQF RTLLFTSEGE KEKRMVDNFR PLQPLMNRTV RSSFRHDYVL NVQAKPKPAT SQATPLEHHH HHH

Entrez

11238

Available Sizes

Frequently Asked Questions

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