ABCB10 Antibody
Goat polyclonal antibody to ABCB10
Product Specifications
Product Name Alternative
Anti ABCB10 antibody, anti ATP-binding cassette, sub-family B (MDR/TAP), member 10 antibody, anti EST20237 antibody, anti M-ABC2 antibody, anti MTABC2 antibody, anti ATP-binding cassette, sub-family B, member 10 antibody, anti ABC-me antibody
Target
ABCB10
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
KPNGIYRKLMNKQ
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:64000, WB: 0.05 μg/ml
Molecular Weight
79.1
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave bands at approx. 90kDa and 50kDa in Human Bone Marrow lysates after 0.05 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 79.1kDa according to NP_036221.2 and given the expected mature size of ~65kDa according to Graf et al. (PMID: 15215243) . Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Human, Mouse, Rat
Tested Applications
ELISA
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
23456
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