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ABCB10 Antibody

Goat polyclonal antibody to ABCB10

Product Specifications

Product Name Alternative

Anti ABCB10 antibody, anti ATP-binding cassette, sub-family B (MDR/TAP), member 10 antibody, anti EST20237 antibody, anti M-ABC2 antibody, anti MTABC2 antibody, anti ATP-binding cassette, sub-family B, member 10 antibody, anti ABC-me antibody

Target

ABCB10

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

KPNGIYRKLMNKQ

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:64000, WB: 0.05 μg/ml

Molecular Weight

79.1

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave bands at approx. 90kDa and 50kDa in Human Bone Marrow lysates after 0.05 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 79.1kDa according to NP_036221.2 and given the expected mature size of ~65kDa according to Graf et al. (PMID: 15215243) . Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Human, Mouse, Rat

Tested Applications

ELISA

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

23456

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