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Frizzled 2 Antibody

Goat polyclonal antibody to FZD2

Product Specifications

Product Name Alternative

Anti FZD2 antibody, anti frizzled homolog 2 (Drosophila) antibody, anti frizzled 2 antibody

Reactivity

Human

Target

Frizzled 2

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

RYATLEHPFHCPR

Field of Research

Stem Cells

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:64000, WB: 0.03-0.1 μg/ml, IHC-P: 2.5 μg/ml

Molecular Weight

63.6

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave an approx. 23kDa band in Human Colon, Heart and Ovary lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 63.6kDa according to NP_001457.1. The 23kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Mouse, Rat

Tested Applications

ELISA, IF, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

2535

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