Frizzled 2 Antibody
Goat polyclonal antibody to FZD2
Product Specifications
Product Name Alternative
Anti FZD2 antibody, anti frizzled homolog 2 (Drosophila) antibody, anti frizzled 2 antibody
Reactivity
Human
Target
Frizzled 2
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
RYATLEHPFHCPR
Field of Research
Stem Cells
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:64000, WB: 0.03-0.1 μg/ml, IHC-P: 2.5 μg/ml
Molecular Weight
63.6
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave an approx. 23kDa band in Human Colon, Heart and Ovary lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 63.6kDa according to NP_001457.1. The 23kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Mouse, Rat
Tested Applications
ELISA, IF, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
2535
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