NRAS Antibody
Goat polyclonal antibody to NRAS
Product Specifications
Product Name Alternative
Anti NRAS antibody, anti neuroblastoma RAS viral (v-ras) oncogene homolog antibody, anti N-ras antibody, anti NRAS1 antibody, anti N-ras protein part 4 antibody, anti v-ras neuroblastoma RAS viral oncogene homolog antibody
Reactivity
Human
Target
NRAS
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
RMKKLNSSDDGTQ
Field of Research
Signal Transduction
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:64000, WB: 0.3-1μg/ml
Molecular Weight
21.2
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Small Intestine shows staining of cytoplasm and membrane of enterocytes. Recommended concentration, 2-3ug/ml.WB: Approx. 21kDa band observed in Human lysates of cell line A431 (calculated MW of 21.2kDa according to NP_002515.1) . In transfected HEK293 transiently expressing NRAS bands of approx. 21kDa and 25kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03 μg/ml.
Prediction Reactivity
Mouse, Rat
Tested Applications
ELISA, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
4893
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