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NRAS Antibody

Goat polyclonal antibody to NRAS

Product Specifications

Product Name Alternative

Anti NRAS antibody, anti neuroblastoma RAS viral (v-ras) oncogene homolog antibody, anti N-ras antibody, anti NRAS1 antibody, anti N-ras protein part 4 antibody, anti v-ras neuroblastoma RAS viral oncogene homolog antibody

Reactivity

Human

Target

NRAS

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

RMKKLNSSDDGTQ

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:64000, WB: 0.3-1μg/ml

Molecular Weight

21.2

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Small Intestine shows staining of cytoplasm and membrane of enterocytes. Recommended concentration, 2-3ug/ml.WB: Approx. 21kDa band observed in Human lysates of cell line A431 (calculated MW of 21.2kDa according to NP_002515.1) . In transfected HEK293 transiently expressing NRAS bands of approx. 21kDa and 25kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03 μg/ml.

Prediction Reactivity

Mouse, Rat

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

4893

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