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RNF34/RFI (internal) Antibody

Goat polyclonal antibody to RNF34

Product Specifications

Product Name Alternative

Anti RNF34 antibody, anti ring finger protein 34 antibody, anti FLJ21786 antibody, anti RFI antibody, anti RIF antibody, anti RIFF antibody, anti FYVE-RING finger protein MOMO antibody, anti RING finger protein RIFF antibody, anti hRFI antibody

Target

RNF34 / RFI (internal)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

RLYKENEENQKSY

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:64000, WB: 0.3 μg/ml

Molecular Weight

41.7; 41.6

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave an approx 35kDa band in Human Lymph Node, Ovary and Testis lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.7kDa according to NP_919247.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Bovine, Canine, Human, Mouse, Rat

Tested Applications

ELISA

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

80196

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