RNF34/RFI (internal) Antibody
Goat polyclonal antibody to RNF34
Product Specifications
Product Name Alternative
Anti RNF34 antibody, anti ring finger protein 34 antibody, anti FLJ21786 antibody, anti RFI antibody, anti RIF antibody, anti RIFF antibody, anti FYVE-RING finger protein MOMO antibody, anti RING finger protein RIFF antibody, anti hRFI antibody
Target
RNF34 / RFI (internal)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
RLYKENEENQKSY
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:64000, WB: 0.3 μg/ml
Molecular Weight
41.7; 41.6
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave an approx 35kDa band in Human Lymph Node, Ovary and Testis lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.7kDa according to NP_919247.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Bovine, Canine, Human, Mouse, Rat
Tested Applications
ELISA
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
80196
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