Calcineurin SubstRate peptide
Calcineurin SubstRate peptide
Product Specifications
Source
Synthetic
Purity
> 95%
Form
Lyophilized powder
Molecular Weight
2112.4
Storage Conditions
Shipped at 4°C. Store at -20°C for one year.
Notes
For research use only.
Applications Notes
The 19-peptide DLDVPIPGRFDRRVSVAAE is a fragment of the RII subunit of the cAMP-dependent protein kinase. It is successfully used in a nonradioactive assay in which both phosphorylation by protein kinase and dephosphorylation by protein phosphatase 2B (calcineurin) are monitored by the simultaneous quantitative determination of phosphorylated and non-phosphorylated peptide using HPLC on an RP18 column with UV detection. This method allows the measurement of enzyme kinetics as well as the characterization of novel inhibitors.
AA Sequence
Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser-Val-Ala-Ala-Glu
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