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Calcineurin SubstRate peptide

Calcineurin SubstRate peptide

Product Specifications

Source

Synthetic

Purity

> 95%

Form

Lyophilized powder

Molecular Weight

2112.4

Storage Conditions

Shipped at 4°C. Store at -20°C for one year.

Notes

For research use only.

Applications Notes

The 19-peptide DLDVPIPGRFDRRVSVAAE is a fragment of the RII subunit of the cAMP-dependent protein kinase. It is successfully used in a nonradioactive assay in which both phosphorylation by protein kinase and dephosphorylation by protein phosphatase 2B (calcineurin) are monitored by the simultaneous quantitative determination of phosphorylated and non-phosphorylated peptide using HPLC on an RP18 column with UV detection. This method allows the measurement of enzyme kinetics as well as the characterization of novel inhibitors.

AA Sequence

Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser-Val-Ala-Ala-Glu

Available Sizes

Frequently Asked Questions

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