Recombinant BMP-2 (Bone morphogenetic protein-2), Human, Animal-Free

Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF)

Human

His-tag at the N-terminus

Escherichia coli

Human

Musculoskeletal & Connective Tissue Research

<0.1 EU per 1 μg of the protein by the LAL method.

>95% as determined by SDS-PAGE. Ni-NTA chromatography.

Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is 3.2 x 106 IU/mg.

Lyophilized

Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.

For research use only.

Cell Culture, ELISA

The protein was lyophilized from a solution containing 20 mM sodium citrate, 0.2 MNaCl, pH 3.5.

650